Age-related changes in the murine cochlear lateral wall
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Updated: Aug 11, 2026

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
Published on: January 1, 2016
1Department of Otolaryngology, Oita Medical University, 1-1 Idaigaoka, Hasama-cho, Oita, Japan. ichimiya@oita-med.ac.jp
Abstract:
Cochleas from C57BL/6 mice were investigated electrophysiologically and histochemically to evaluate the pathology of presbycusis. The average auditory brainstem response thresholds from 6-week-old mice were significantly lower than those of 6-month-old mice and those of 1-year-old mice. Histologic observation revealed changes in the cochlea after age 6 months. Conventional hematoxylin and eosin (H&E) staining showed disorganization of the organ of Corti, a decrease in the number of spiral ganglion cells, and atrophy of the stria vascularis. Although H&E staining and type II collagen immunolabeling did not show obvious changes in the spiral ligament (SL), the density of connexin 26 staining was reduced in this region. Sodium-potassium-adenosinetriphosphatase immunolabeling was increased in the SL, whereas its average density was not significantly altered in the stria vascularis. These results suggest that the SL could be among the regions responsible for cochlear malfunction with aging.
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