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Related Experiment Video

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Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses

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Particle-mediated DNA immunization of cattle confers long-lasting immunity against bovine herpesvirus-1.

R P Braun¹, L A Babiuk, B I Loehr

¹Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan, S7N 5E3, Canada.

|December 22, 1999

Summary

Particle-mediated delivery effectively vaccinated ruminants using a DNA vaccine. Gene gun delivery demonstrated long-lasting immune responses and protection against bovine herpesvirus-1 in cattle.

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Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Published on: May 19, 2023

Area of Science:

Veterinary Immunology
Molecular Biology
Biotechnology

Background:

Particle-mediated delivery, specifically gene gun technology, is an emerging method for DNA vaccine administration.
Understanding the cellular response and transfection efficiency is crucial for optimizing this delivery system in target species.
Bovine herpesvirus-1 (BHV-1) poses a significant threat to cattle health, necessitating effective vaccination strategies.

Purpose of the Study:

To optimize gene gun parameters for DNA vaccine delivery into ruminant skin.
To characterize the cellular response to particle delivery and identify transfected cells.
To evaluate the efficacy of gene gun-delivered DNA vaccination against BHV-1 in cattle.

Main Methods:

Optimized gene gun parameters for gold particle delivery into the bovine epidermis.
Utilized a plasmid encoding green fluorescent protein (GFP) to visualize transfected cells.
Administered a DNA vaccine encoding a truncated BHV-1 glycoprotein D via gene gun to cattle.
Assessed immune responses including serum antibodies, lymphocyte proliferation, and interferon-gamma secretion.
Challenged vaccinated cattle with BHV-1 to determine vaccine efficacy.

Main Results:

Gold particle delivery induced an inflammatory response, independent of plasmid presence.
Transfected cells expressing GFP were primarily located in superficial epidermal layers.
Gene gun immunization elicited robust, long-lasting serum antibody and cellular immune responses.
Vaccinated cattle demonstrated significant protection against BHV-1 challenge.

Conclusions:

Gene gun-based particle-mediated delivery is an effective method for DNA vaccination in cattle.
The induced immune responses provide durable protection against bovine herpesvirus-1.
This technology holds promise for developing novel vaccines for ruminant species.