Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid solubilization in human gallbladder versus hepatic biles.

E R Eckhardt1, K J van Erpecum, M B de Smet

  • 1Dept. of Gastroenterology and Surgery, University Hospital, Utrecht, The Netherlands.

Journal of Hepatology
|December 22, 1999
PubMed
Summary

Gallbladder bile has fewer cholesterol and phospholipid vesicles than liver bile. Supersaturated mixed micelles in gallbladder bile suggest non-vesicular cholesterol crystallization may occur.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intra-gastric balloon with lifestyle modification: a promising therapeutic option for overweight and obese patients with metabolic dysfunction-associated steatotic liver disease.

Internal and emergency medicine·2023
Same author

Stauffer's syndrome: A true entity?

Clinics and research in hepatology and gastroenterology·2021
Same author

Retrieval of chronic hepatitis C patients. A manifesto for action to eliminate hepatitis C in the Netherlands: the CELINE project.

The Netherlands journal of medicine·2019
Same author

Strategies for achieving viral hepatitis C micro-elimination in the Netherlands.

Hepatology, medicine and policy·2018
Same author

Ribavirin steady-state plasma level is a predictor of sustained virological response in hepatitis C-infected patients treated with direct-acting antivirals.

Alimentary pharmacology & therapeutics·2017
Same author

How the concept of biochemical response influenced the management of primary biliary cholangitis over time.

The Netherlands journal of medicine·2016

Area of Science:

  • Bile acid and lipid metabolism research
  • Gastroenterology and hepatology
  • Cholesterol gallstone disease pathogenesis

Background:

  • Gallbladder bile concentrates lipids, potentially altering cholesterol crystallization dynamics.
  • Cholesterol crystallization in bile is a key step in gallstone formation.
  • Existing theories suggest supersaturated vesicles drive rapid crystallization in gallbladder bile.

Purpose of the Study:

  • To compare lipid solubilization and phase behavior in hepatic versus gallbladder bile.
  • To investigate the role of vesicular and micellar lipid fractions in cholesterol crystallization.
  • To determine if gallbladder bile concentration alters lipid vesicle formation.

Main Methods:

  • Separation of mixed micellar and vesicular lipid phases from patient bile samples.

Related Experiment Videos

  • Utilized advanced gel filtration chromatography for phase separation.
  • Analyzed lipid composition and concentrations in hepatic and gallbladder bile.

    • Main Results:

    • Vesicles were significantly less abundant in gallbladder bile compared to hepatic bile (2/7 vs. 6/7 patients).
    • Vesicular cholesterol and phospholipid content (percentage and mM) were markedly reduced in gallbladder bile.
    • Gallbladder bile mixed micelles exhibited a higher cholesterol saturation index, with supersaturation observed in the absence of vesicles.

    Conclusions:

    • Bile concentration in the gallbladder reduces vesicular lipid content.
    • Supersaturated mixed micelles in gallbladder bile, without vesicles, suggest alternative crystallization pathways.
    • Findings challenge the sole reliance on vesicular mechanisms for cholesterol crystallization in gallstone disease.