Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automatic quantification of microvessel density in urinary bladder carcinoma.

K Wester1, P Ranefall, E Bengtsson

  • 1Department of Genetics & Pathology, Uppsala University, Sweden.

British Journal of Cancer
|December 22, 1999
PubMed
Summary

This study evaluated microvessel density (MVD) in bladder cancer biopsies using automated and semi-automated methods. Automated quantification is reliable for microvessel number and area when staining is high quality.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MR-guided adaptive versus ITV-based stereotactic body radiotherapy for hepatic metastases (MAESTRO): a randomized controlled phase II trial.

Radiation oncology (London, England)·2022
Same author

The novel collagen matrikine, endotrophin, is associated with mortality and cardiovascular events in patients with atherosclerosis.

Journal of internal medicine·2021
Same author

Activation of immune responses against the basement membrane component collagen type IV does not affect the development of atherosclerosis in ApoE-deficient mice.

Scientific reports·2019
Same author

A biomarker of collagen type I degradation is associated with cardiovascular events and mortality in patients with atherosclerosis.

Journal of internal medicine·2018
Same author

Two-step kinetic model of the self-assembly mechanism for diphenylalanine micro/nanotube formation.

Physical chemistry chemical physics : PCCP·2017
Same author

Autoantibodies against aldehyde-modified collagen type IV are associated with risk of development of myocardial infarction.

Journal of internal medicine·2017

Area of Science:

  • Uro-oncology
  • Pathology
  • Medical image analysis

Background:

  • Accurate assessment of microvessel density (MVD) is crucial for bladder carcinoma prognosis.
  • Traditional manual assessment of MVD is subjective and time-consuming.
  • Developing objective and reproducible quantification methods is essential.

Purpose of the Study:

  • To evaluate semi-automatic and automatic image analysis techniques for quantifying MVD in TUR-T bladder cancer biopsies.
  • To compare biopsy results with cystectomy specimens.
  • To assess the reproducibility and observer variability of MVD quantification.

Main Methods:

  • Seventy-three TUR-T bladder carcinoma biopsies were analyzed.
  • Immunostainings were performed using an optimized protocol.

Related Experiment Videos

  • Microvessel density was quantified by microvessel number (nMVD) and endothelial cell area (aMVD) using semi-automatic and automatic image analysis.
  • Comparison with 12 corresponding cystectomy specimens was conducted.

    • Main Results:

    • A good correlation (92%) was observed between biopsy and cystectomy specimens.
    • Both semi-automatic and automatic techniques demonstrated reproducibility for nMVD and aMVD quantification relative to total tumor area.
    • Automatic quantification accuracy was contingent on high-quality immunostaining.
    • Subjective visual estimation of MVD showed poor observer reliability.

    Conclusions:

    • Quantification of MVD relative to a single tissue compartment is proposed.
    • Automatic quantification is a viable and efficient method for MVD assessment in bladder cancer when immunostaining quality is high.
    • Automated methods minimize selection variability by enabling quantification of multiple areas.