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Related Experiment Videos

Estrogen activates telomerase.

S Kyo1, M Takakura, T Kanaya

  • 1Department of Obstetrics and Gynecology, School of Medicine, Kanazawa University, Ishikawa, Japan. satoruky@med.kanazawa-u.ac.jp

Cancer Research
|December 22, 1999
PubMed
Summary
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Estrogen up-regulates telomerase activity in cancer cells by directly binding to the hTERT promoter and indirectly via c-Myc. This sheds light on hormonal control of cell division and estrogen

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Cancer Research

Background:

  • Telomerase activity is crucial for the unlimited proliferation of cancer cells.
  • Mechanisms regulating telomerase, particularly hormonal influences, are not fully understood.
  • Estrogen's role in cancer progression is significant, but its direct impact on telomerase needs clarification.

Purpose of the Study:

  • To investigate the effects of estrogen on telomerase activity in estrogen receptor-positive breast cancer cells.
  • To elucidate the molecular mechanisms by which estrogen influences telomerase gene expression.

Main Methods:

  • Treatment of MCF-7 cells with 17beta-estradiol.
  • Analysis of telomerase activity and human telomerase reverse transcriptase (hTERT) mRNA levels.

Related Experiment Videos

  • Gel shift assays to assess estrogen receptor (ER) binding to the hTERT promoter.
  • Luciferase reporter assays to determine the functional role of the estrogen-responsive element (ERE) in the hTERT promoter.
  • Investigation of c-Myc expression and its binding to E-boxes in the hTERT promoter.

    • Main Results:

    • 17beta-estradiol significantly increased telomerase activity and hTERT mRNA levels in MCF-7 cells.
    • Estrogen receptor (ER) directly binds to an imperfect palindromic ERE in the hTERT promoter, mediating transcriptional activation.
    • Estrogen also up-regulates c-Myc, which further contributes to estrogen-induced hTERT transactivation via E-boxes.

    Conclusions:

    • Estrogen activates telomerase in ER-positive cancer cells through both direct and indirect mechanisms involving the hTERT promoter.
    • These findings reveal a novel pathway for hormonal regulation of telomerase.
    • Understanding this mechanism may offer insights into cellular senescence, aging, and estrogen-driven carcinogenesis.