Related Experiment Videos
Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins
S K Lyman1, L Gerace, S J Baserga
1Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
RNA (New York, N.Y.)
|December 22, 1999
Abstract:
We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.