Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conformational transition in DNA on a cold surface.

X Z Feng1, R Bash, P Balagurumoorthy

  • 1Department of Physics, Arizona State University, Tempe, AZ 85287-1504, USA.

Nucleic Acids Research
|December 22, 1999
PubMed
Summary

DNA fragments contract on surfaces at low temperatures, likely due to reduced water activity. This DNA contraction phenomenon is dependent on both temperature and surface interaction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design of a novel antisymmetric coil array for parallel transmit cardiac MRI in pigs at 7 T.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2019
Same author

[Clinical features and advanced progress of secondary BPPV].

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery·2018
Same author

The Effect of Palp Loss on Feeding Behavior of Two Spionid Polychaetes: Changes in Exposure.

The Biological bulletin·2018
Same author

Process-Specific Recruitment Cues in Marine Sedimentary Systems.

The Biological bulletin·2017
Same author

Measles virus spread initiated at international mass gatherings in Europe, 2011.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2014
Same author

Characterization of the alfalfa (Medicago sativa) genome by DNA reassociation.

Plant molecular biology·2013

Area of Science:

  • Biophysics
  • Materials Science
  • Molecular Biology

Background:

  • Understanding DNA behavior on surfaces is crucial for nanotechnology and molecular diagnostics.
  • DNA conformation is influenced by environmental factors like temperature and substrate interactions.

Purpose of the Study:

  • To investigate the effect of deposition temperature on the contour length of DNA fragments adsorbed onto surfaces.
  • To determine the conditions under which DNA molecules exhibit significant conformational changes.

Main Methods:

  • Measuring the contour length of DNA fragments deposited on mica substrates under buffer conditions.
  • Utilizing techniques to image DNA molecules on both bare and treated surfaces (Ni- and silane-treated).
  • Comparing DNA behavior on surfaces versus in solution at varying temperatures (2°C and 25°C).

Related Experiment Videos

Main Results:

  • Extended DNA molecules on treated surfaces rapidly contract as temperature decreases, reaching the contour length of A-DNA at 2°C.
  • This temperature-dependent contraction is sequence-independent.
  • DNA contraction was not observed in solution at 2°C or on surfaces at 25°C.

Conclusions:

  • DNA contraction on surfaces is a combined effect of low temperature and surface contact.
  • Reduced water activity at a cold surface is the probable cause for DNA conformational changes.
  • Surface properties and temperature are critical parameters influencing DNA structure and behavior.