Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Bone marrow cell trafficking following intravenous administration.

J Cui¹, R L Wahl, T Shen

¹Howard Hughes Medical Institute, Department of Human Genetics, Ann Arbor, MI, USA.

British Journal of Haematology

|December 22, 1999

Summary

Transplanted bone marrow cells rapidly distribute to various tissues, including the bone marrow, within hours of infusion. This suggests marrow engraftment may involve non-specific seeding rather than specific homing signals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[A case of highly differentiated adenocarcinoma of gastric type with special morphology].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2020

Same author

High expression of LINC0163 promotes progression of papillary thyroid cancer by regulating epithelial-mesenchymal transition MITF.

European review for medical and pharmacological sciences·2020

Same author

[Value of left ventricular myocardial strain derived from cardiac magnetic resonance tissue tracking on differentiating constrictive pericarditis from restrictive cardiomyopathy].

Zhonghua xin xue guan bing za zhi·2020

Same author

<i>Porphyromonas gingivalis</i> Promotes Oral Squamous Cell Carcinoma Progression in an Immune Microenvironment.

Journal of dental research·2020

Same author

Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020

Same author

EZH2-mediated H3K27me3 enrichment on the lncRNA MEG3 promoter regulates the growth and metastasis of glioma cells by regulating miR-21-3p.

European review for medical and pharmacological sciences·2020

Area of Science:

Hematology
Transplantation Biology
Cell Trafficking

Background:

Understanding the immediate distribution of transplanted bone marrow cells is crucial for improving engraftment efficiency.
Current models lack clarity on whether transplanted cells exhibit specific homing to the bone marrow.

Purpose of the Study:

To investigate the early trafficking and distribution of infused donor bone marrow cells in a syngeneic mouse model.
To determine if transplanted cells exhibit specific homing to the bone marrow or if distribution is non-specific.

Main Methods:

Utilized three distinct cell-marking techniques: indium-111 oxine (111In), PKH26 fluorescent dye, and a transgene marker.
Evaluated donor cell presence in various tissues, including peripheral blood, at 3-48 hours post-infusion.

Related Experiment Videos

Compared cell localization in non-myeloablated versus irradiated recipients and assessed homing in secondary recipients.

Main Results:

Infused donor cells rapidly distributed to bone marrow, liver, spleen, and muscle within 4 hours, persisting for 48 hours.
Approximately 17% of cells localized to the marrow in non-irradiated recipients, compared to 1-2% in irradiated recipients.
Recovered cells from a first host and re-infused into a secondary host did not show increased marrow homing.

Conclusions:

Early marrow engraftment may result from non-specific 'seeding' of cells rather than a specific homing mechanism.
Efficient targeting of transplanted cells to the bone marrow is a complex process influenced by multiple factors.