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[Effect of urea on proteolysis of histone H2A in calf thymus nuclei]
V I Tiulenev1, L A Konoplich, O A Rudenko
1Taras Shevchenko Kyiv University.
Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
|December 28, 1999
Abstract:
The 1 hour incubation of calf thymus nuclei at 37 degrees C leads to a proteolysis of the histones H1, H3 and H2B. Urea does not influence the histone degradation while 1.5 and 2.0 M NaCl lead to the proteolysis of the H2A histone. On this background, 2 M urea restrains the degradation of the H2A histone. It is assumed that hydrogen bonds are very important for the activity of the proteinases and its interaction with the H2A histone.