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Related Experiment Videos

Oxidative stress in patients with multiple sclerosis.

C Syburra1, S Passi

  • 1Dermatological Institute, Rome, Italy. thsyburr@studi.unizh.ch

Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
|December 28, 1999
PubMed
Summary

Multiple sclerosis patients exhibit lower blood antioxidant levels, including Vitamin E and ubiquinone, alongside higher polyunsaturated fatty acids, indicating significant oxidative stress. This suggests antioxidant supplementation and diet may help manage MS progression.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Oxidative Stress Research

Background:

  • Nervous system cells are susceptible to oxidative damage due to low antioxidant content and high polyunsaturated fatty acids.
  • Multiple Sclerosis (MS) is a neurological disease where oxidative stress is a significant factor.

Purpose of the Study:

  • To investigate and quantify oxidative stress markers in the blood of multiple sclerosis patients compared to healthy controls.
  • To analyze the levels of various enzymatic and non-enzymatic antioxidants and fatty acid profiles in MS patients.

Main Methods:

  • Multiparameter analysis of antioxidants: Vitamin E, ubiquinone, glutathione, superoxide dismutase, glutathione peroxidase, catalase.
  • Fatty acid patterns of phospholipids analyzed using Gas Chromatography-Mass Spectrometry (GC-MS).

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  • Ubiquinone and glutathione levels measured by High-Performance Liquid Chromatography (HPLC); enzyme activities by spectrophotometry.

    • Main Results:

    • MS patients showed significantly reduced levels of plasma ubiquinone and Vitamin E, and erythrocyte glutathione peroxidase compared to controls.
    • Lymphocyte ubiquinone levels were also significantly lower in MS patients.
    • Elevated plasma levels of phospholipid polyunsaturated fatty acids (PL-PUFA), particularly C20:3 n-6 and C20:4 n-6, were observed in MS patients.

    Conclusions:

    • The blood of multiple sclerosis patients displays clear indicators of significant oxidative stress.
    • Antioxidant deficiency and increased lipid peroxidation are implicated in MS pathogenesis.
    • Targeted antioxidant therapy, including Vitamin E, ubiquinol, and selenium, alongside dietary modifications, may offer a promising strategy to mitigate MS progression.