Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical service organisation for adults with atrial fibrillation: Cochrane systematic review and meta-analysis.

European journal of cardiovascular nursing·2025
Same author

Anaesthetic management of obstetric patients with Chiari type I malformation: a retrospective case series and literature review.

International journal of obstetric anesthesia·2024
Same author

A unified call to action from Australian nursing and midwifery leaders: ensuring that Black lives matter.

Contemporary nurse·2020
Same author

"Two heads are better than one"- pharmacy and nursing students' perspectives on interprofessional collaboration utilizing the RIPE model of learning.

Research in social & administrative pharmacy : RSAP·2019
Same author

Real-world Outcomes and Factors Predicting Survival and Completion of Radium 223 in Metastatic Castrate-resistant Prostate Cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2018
Same author

Development of the RIPE model (Reflective Interprofessional Education Model) to enhance interprofessional collaboration.

Research in social & administrative pharmacy : RSAP·2018

Related Experiment Video

Updated: Jul 9, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays.

P S Nelson1, V Hawkins, M Schummer

  • 1Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA. psnels@u.washington.edu

Genetic Analysis : Biomolecular Engineering
|December 28, 1999
PubMed
Summary

Researchers developed a novel negative hybridization selection method to reduce redundancy in cDNA libraries. This technique enhances the diversity of identified genes, improving the efficiency of gene discovery for functional studies.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Related Experiment Videos

Last Updated: Jul 9, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Identifying all expressed genes (the complement) is crucial for understanding biological functions.
  • Large-scale sequencing of complementary DNA (cDNA) libraries is a common gene discovery method.
  • This method suffers from high redundancy due to varying gene transcription levels, leading to duplicated clones.

Purpose of the Study:

  • To develop a method for reducing redundancy in cDNA libraries.
  • To increase the diversity of identified expressed genes.
  • To improve the efficiency of gene discovery for functional genomics.

Main Methods:

  • A negative hybridization selection strategy was developed.
  • Complex cDNA probes were hybridized to high-density cDNA clone arrays.
  • Clones with null or low hybridization signals were selected.

Main Results:

  • Applied to a human prostate cDNA library, the method reduced highly expressed prostate cDNAs from 6.8% to 0.57%.
  • Overall clone redundancy decreased from 33% to 11%.
  • The diversity of the selected cDNA population increased, with 89% distinct cDNAs compared to 67% in randomly selected clones.

Conclusions:

  • Negative hybridization selection effectively reduces cDNA library redundancy and increases transcript diversity.
  • This method offers advantages over traditional normalization techniques, including flexibility with existing libraries and iterative screening for rare transcripts.
  • The improved cDNA representation facilitates more comprehensive gene discovery and functional studies.