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Updated: Jul 9, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Site-directed insertion and insertion-deletion mutations in the Escherichia coli chromosome simplified
1Biochemistry Department, Michigan State University, East Lansing 48824-1319, USA. smithwhi@pilot.msu.edu
Abstract:
A procedure to produce an exact chromosomal replica of an insertion or insertion-deletion mutation produced in vitro in a plasmid with a ColE 1 origin of replication is presented. This procedure uses a previously described property of recD mutations (Biek DP, Cohen SN. J. Bacteriol. 1986;167:594-603) and is limited by (1) the compatibility of the new mutation with recD; and (2) the presence of some Escherichia coli DNA flanking the mutation.
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