Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of membrane halves: cholesterol.

K A Fisher

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1976
    PubMed
    Summary

    Freeze-fracture of human erythrocyte membranes revealed cholesterol asymmetry. This technique separates outer and inner membrane halves for chemical analysis, showing more cholesterol on the exterior side.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    An evaluation of the Chesapeake Bay management strategy to improve water quality in small agricultural watersheds.

    Journal of environmental management·2021
    Same author

    Multichannel deconvolution of vibrational signals: A state-space inverse filtering approach.

    The Journal of the Acoustical Society of America·2021
    Same author

    Transient recovery problem in acoustics: A multichannel model-based deconvolution approach.

    The Journal of the Acoustical Society of America·2021
    Same author

    Multichannel spectral estimation in acoustics: A state-space approach.

    The Journal of the Acoustical Society of America·2020
    Same author

    Vibrational processing of a dynamic structural flight system: A multichannel spectral estimation approach.

    The Journal of the Acoustical Society of America·2020
    Same author

    Multichannel processing of vibrational measurements: A constrained subspace application.

    The Journal of the Acoustical Society of America·2019

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Membrane Biophysics

    Background:

    • Freeze-fracture is a preparative technique for analyzing membrane halves.
    • Previous work demonstrated nonrandom fracture patterns in erythrocyte monolayers.
    • Erythrocyte monolayers bound to cationized glass yield enriched membrane fractions.

    Purpose of the Study:

    • To quantify cholesterol distribution in human erythrocyte membranes.
    • To utilize microanalysis compatible cell quantities.
    • To determine fractional enrichment of outer and inner membrane halves.

    Main Methods:

    • Freeze-fracture of human erythrocyte monolayers.
    • Quantitative thin-layer chromatography for nanogram cholesterol assay.
    • Lipid extraction from intact and fractured membranes.

    Main Results:

    • Cholesterol was found to be asymmetrically distributed in the erythrocyte membrane.
    • A higher concentration of cholesterol was detected on the exterior membrane side compared to the interior.
    • The freeze-fracture method allowed for the analysis of lipid asymmetry.

    Conclusions:

    • Human erythrocyte membranes exhibit significant cholesterol asymmetry.
    • Freeze-fracture is a viable method for investigating lipid distribution in membrane leaflets.
    • The exterior leaflet of the erythrocyte membrane is enriched in cholesterol.

    Related Experiment Videos