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Related Experiment Videos

Mice cloned from embryonic stem cells.

T Wakayama1, I Rodriguez, A C Perry

  • 1Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI 96822, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 28, 1999
PubMed
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Scientists successfully cloned mice from established embryonic stem (ES) cell lines. This breakthrough links ES cell technology with animal cloning, enabling the production of viable cloned mice from late-passage cells.

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Genetics

Background:

  • Cloning by nuclear transfer has historically relied on freshly isolated or primary cells.
  • Previous work demonstrated successful cloning of mice from adult somatic cells.
  • Established embryonic stem (ES) cell lines, particularly at late passage, were unexplored for cloning.

Purpose of the Study:

  • To investigate the feasibility of cloning mice using established embryonic stem (ES) cell lines at late passage.
  • To determine if ES cell nuclei can support full embryonic development after nuclear transfer.
  • To link ES cell technology with advancements in animal cloning.

Main Methods:

  • Nuclear transfer by microinjection using established ES cell lines (R1 and E14) at late passage.

Related Experiment Videos

  • In vitro development assessment of reconstructed oocytes to the morula/blastocyst stage.
  • Transfer of cloned embryos to surrogate mothers for assessment of live-born offspring development.

    • Main Results:

    • 29% of reconstructed oocytes using R1 ES cells developed to the morula/blastocyst stage in vitro.
    • 8% of these embryos resulted in live-born cloned pups, with 26 mice cloned from R1 cells.
    • ES cell nuclei from G(1)- or G(2)/M-phases were shown to efficiently support full development to term.

    Conclusions:

    • Late-passage embryonic stem (ES) cells can be successfully used to produce viable cloned mice.
    • This study establishes a significant connection between ES cell technology and animal cloning.
    • The findings suggest the potential for cloning numerous individuals from a single ES cell over time.