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Investigation of a tetracycline-regulated phage display system
1Institut für Biochemie (Charité), Humboldt-Universität zu Berlin, Monbijoustr.2, D-10117 Berlin, Germany.
Protein Engineering
|December 28, 1999
Abstract:
A new vector (pGZ1) was developed for bacterial phage display of antibody fragments using a transcriptional regulation element with tight control. The tet(p/) degrees -based phasmid exhibits fully suppressed scFv background synthesis in the absence of inducer and is independent of glucose as a catabolite repressor. The vector is shown to be a useful alternative to commonly used lac(p/) degrees -regulated systems.