Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antimicrobial activity of polycationic peptides.

A Giacometti1, O Cirioni, F Barchiesi

  • 1Institute of Infectious Diseases and Public Health, University of Ancona, Italy. cmalinf@popcsi.unian.it

Peptides
|December 28, 1999
PubMed
Summary

Six polycationic peptides show potent antimicrobial activity against various pathogens. These peptides demonstrate rapid, time-dependent killing, with varying efficacy against gram-positive bacteria, gram-negative bacteria, yeasts, and Pneumocystis carinii.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Acceptability of innovative male contraceptive methods among major of age heterosexual adult men in France in 2021].

Progres en urologie : journal de l'Association francaise d'urologie et de la Societe francaise d'urologie·2023
Same author

Corrigendum to "How to: EUCAST recommendations on the screening procedure E.Def 10.1 for the detection of azole resistance in Aspergillus fumigatus isolates using four-well azole-containing agar plates" [Clin Microbiol Infect 25 (2019) 681-687].

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2023
Same author

Is there a POST-COVID dermatological syndrome? The integrated dermato-infectious disease experience of a single centre.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Tocilizumab in COVID-19 interstitial pneumonia.

Journal of internal medicine·2021
Same author

How to: EUCAST recommendations on the screening procedure E.Def 10.1 for the detection of azole resistance in Aspergillus fumigatus isolates using four-well azole-containing agar plates.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2018
Same author

Letters.

The Physician and sportsmedicine·2016

Area of Science:

  • Antimicrobial Peptides
  • Microbiology
  • Infectious Diseases

Background:

  • Polycationic peptides are a class of antimicrobial agents with broad-spectrum activity.
  • Understanding the specific activity profiles of different peptides is crucial for therapeutic development.
  • Clinical isolates present a relevant model for evaluating antimicrobial efficacy.

Purpose of the Study:

  • To evaluate the in vitro antimicrobial activity of six polycationic peptides: buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin.
  • To determine the efficacy of these peptides against a range of clinical isolates, including bacteria, yeasts, Pneumocystis carinii, and Cryptosporidium parvum.
  • To characterize the killing kinetics and spectrum of activity for each peptide.

Main Methods:

Related Experiment Videos

  • Microbroth dilution assays were employed to determine the minimum inhibitory concentrations (MICs) of the peptides.
  • The study utilized clinical isolates of gram-positive and gram-negative aerobic bacteria.
  • Evaluations included activity against yeasts (Candida spp.), Pneumocystis carinii, and Cryptosporidium parvum.

    • Main Results:

    • Peptides displayed differential antimicrobial activities and rapid, time-dependent killing kinetics.
    • Buforin II and cecropin P1 were most effective against gram-negative bacteria.
    • Buforin II and ranalexin showed highest activity against gram-positive strains; ranalexin was most potent against Candida spp.; magainin II excelled against Pneumocystis carinii.

    Conclusions:

    • Polycationic peptides exhibit significant in vitro activity against a variety of microbial pathogens.
    • The study highlights the distinct antimicrobial profiles of individual peptides, suggesting potential for targeted applications.
    • While effective against bacteria, yeasts, and Pneumocystis, these peptides showed limited activity against Cryptosporidium parvum.