Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel method for demonstrating nuclear contribution in mouse nuclear transfer.

M Munsie1, T Peura, A Michalska

  • 1Centre for Early Human Development, Institute of Reproduction and Development, Monash University, Vic., Australia.

Reproduction, Fertility, and Development
|December 28, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of endoscope cleaning and disinfection efficacy, and the impact of endoscope storage on the microbiological safety level.

Journal of applied microbiology·2019
Same author

Improving access to ART in low-income settings through knowledge transfer: a case study from Zimbabwe.

Human reproduction open·2019
Same author

Interoceptive accuracy and interoceptive sensibility in individuals with alcohol use disorder-Different phenomena with different clinical correlations?

Drug and alcohol dependence·2019
Same author

Enzyme assays using sensor arrays based on ion-selective carbon nanotube field-effect transistors.

Biosensors & bioelectronics·2016
Same author

Selective ion-sensing with membrane-functionalized electrolyte-gated carbon nanotube field-effect transistors.

The Analyst·2014
Same author

Imidazole-induced contractility of vascular smooth muscle cells in the presence of U-73122, ODQ, indomethacin and 7-nitroindazole.

Polish journal of veterinary sciences·2013
Same journal

Bifidobacterium longum BB536 supplementation is associated with increased circulating choline plasmalogen concentrations in non-pregnant, non-lactating dairy cows.

Reproduction, fertility, and development·2026
Same journal

Interleukin 12 on extracellular matrix-related factors and functional characteristics of equine endometrial fibroblasts.

Reproduction, fertility, and development·2026
Same journal

CRH expression and function in mouse uterus during early pregnancy.

Reproduction, fertility, and development·2026
Same journal

Corrigendum to: Influence of neonatal estrogens on rat prostate development.

Reproduction, fertility, and development·2026
Same journal

Cellular and molecular characterization of the testicular development of GDF9-edited pigs.

Reproduction, fertility, and development·2026
Same journal

Comparative analysis of acidosis defense mechanisms in preimplantation embryos in BALB/c strain mice: in vivo vs in vitro development.

Reproduction, fertility, and development·2026
See all related articles

This study introduces a novel method using lacZ transgenic mice to confirm nuclear contribution in nuclear transfer embryos and offspring. This technique provides a clear visual marker for nuclear origin in reproductive technologies.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Confirming nuclear contribution is critical for nuclear transfer experiments.
  • Demonstrating nuclear contribution in early embryos is challenging.
  • lacZ transgenic mice offer a potential solution for visualizing nuclear origin.

Purpose of the Study:

  • To establish a reliable method for confirming nuclear contribution in nuclear transfer embryos and offspring.
  • To utilize lacZ expression as a distinct marker for nuclear origin.
  • To validate the totipotency of nuclear transfer blastocysts.

Main Methods:

  • Generated nuclear transfer embryos using metaphase-arrested nuclei from lacZ transgenic (Zin40) and non-transgenic 4-cell embryos.
  • Utilized X-gal staining to detect lacZ expression, indicated by blue nuclei.

Related Experiment Videos

  • Confirmed blastocyst totipotency through the generation of live-born offspring.

    • Main Results:

    • Transgenic nuclear transfer embryos, fetuses, and pups exhibited distinctive blue nuclei after X-gal staining.
    • The blue nuclei pattern directly correlated with the transgenic origin of donor nuclei.
    • Successfully confirmed nuclear contribution in both embryos and live offspring.

    Conclusions:

    • lacZ transgenic mice provide an effective and straightforward marker for confirming nuclear contribution in nuclear transfer.
    • This method simplifies the assessment of nuclear origin in nuclear transfer embryos and progeny.
    • The marker facilitates further investigation into the developmental potential of nuclear transfer embryos, including chimaeric embryo analysis.