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Aptamers containing thymidine 3'-O-phosphorodithioates: synthesis and binding to nuclear factor-kappaB
X Yang1, S Fennewald, B A Luxon
1Sealy Center for Structural Biology, Department of Human Biological Chemistry & Genetics, The University of Texas Medical Branch at Galveston, 77555-1157, USA.
Bioorganic & Medicinal Chemistry Letters
|December 28, 1999
Abstract:
Aptamers targeting NF-kappaB containing thymidine 3'-O-phosphorodithioates in selected positions of an oligonucleotide duplex were synthesized. Binding affinities to NF-kappaB varied with the number and positions of the dithioate backbone substitutions. One of the aptamers showed specific binding to a single NF-kappaB dimer in cell culture extracts.