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Dicistronic Gene Expression in Developing Zebrafish.

Fahrenkrug1, Clark, Dahlquist

  • 1Department of Genetics, Cell Biology and Development, and Institute of Human Genetics, University of Minnesota, Saint Paul, MN 55108-1095, U.S.A.

Summary

Internal ribosome entry sites (IRESs) enable gene expression from single mRNA. The encephalomyocarditis virus (EMCV) IRES successfully drove gene expression in zebrafish embryos, proving its utility in vertebrate systems.

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