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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

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Accessory cells with immunophenotypic and functional features of monocyte-derived dendritic cells are recruited to

A M Tager1, A D Luster, C P Leary

  • 1Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.

Journal of Leukocyte Biology
|December 30, 1999
PubMed

Insights

Inflammatory lung injury from bleomycin (BLM) causes an increase in pulmonary macrophages exhibiting dendritic cell (DC) features. These monocyte-derived DCs appear in the blood and lungs, suggesting a role in inflammatory responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Pulmonary Medicine

Background:

  • Pulmonary macrophages increase during bleomycin-induced lung inflammation.
  • Exudate macrophages in bleomycin lung injury show dendritic cell (DC) characteristics.

Purpose of the Study:

  • To characterize the phenotype and function of inflammatory cells in bleomycin-induced lung injury.
  • To investigate the differentiation of macrophages into dendritic cells during lung inflammation.

Main Methods:

  • Intratracheal administration of bleomycin (BLM) in C57BL/6 mice.
  • Characterization of bronchoalveolar lavage fluid (BAMC) cells for morphology, immunophenotype (MHC class II, 33D1, Factor XIIIa, CD80, CD86), and accessory cell function (MLR).
  • Analysis of peripheral blood monocytes post-BLM treatment.

Main Results:

  • At day 7 post-BLM, 48% of CD11b+ BAMC exhibited DC features, including dendritic morphology and expression of DC markers.
  • These cells demonstrated the ability to stimulate allogeneic lymphocyte responses (MLR).
  • CD11b+ peripheral blood monocytes also showed increased expression of DC-associated markers and enhanced MLR stimulatory capacity after BLM treatment.

Conclusions:

  • Inflammatory dendritic cells (DCs) with monocyte-derived features increase in the lungs and peripheral blood following an inflammatory stimulus.
  • These findings highlight a potential role for monocyte-derived DCs in bleomycin-induced lung injury and inflammation.

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