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Updated: Jun 15, 2026

Calcium Imaging In Electrically Stimulated Flat-Mounted Retinas
Published on: August 18, 2023
The expression of Ca2+/calmodulin-dependent protein kinase I in rat retina is regulated by light stimulation
T Tsumura1, A Murata, F Yamaguchi
1Department of Ophthalmology, Kagawa Medical University, Japan.
Abstract:
Ca2+/calmodulin-dependent protein kinase I (CaM-kinase I) in rat retina was analyzed by immunohistochemical analysis, Western blot analysis and kinase activity assay. Western blot analysis revealed two immunoreactive bands similar to those detected in the brain. Developmental studies revealed that CaM-kinase I expression increased in accordance with postnatal development. Expression of CaM-kinase I in the retinas of rats raised in the complete darkness markedly decreased. CaM-kinase I activity assay supported these findings. Synapsin I was shown to be a possible intrinsic substrate of CaM-kinase I in rat retina. These results elucidated that CaM-kinase I is expressed in the retina and may play an important role in the retinal functions and that the expression of CaM-kinase I is regulated by light stimulation.
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