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Chiral separation of beta-blockers after derivatization with (-)-menthyl chloroformate by reversed-phase high
1College of Pharmacy, Kangwon National University, Chunchon, Korea. kyeong@cc.kangwon.ac.kr
Abstract:
Optimum conditions of chiral derivatization reaction of beta-blockers acebutolol, arotinolol, beta-xolol, bisoprolol, celiprolol, metoprolol and pindolol) with (-)-menthyl chloroformate were investigated for the resolution by HPLC. With more than 30 times molar excess of (-)-menthyl chloroformate chiral derivatization reactions were completed within one hour at room temperature except arotinolol and celiprolol. Diastereomeric derivatives of beta-blockers were well resolved on the ODS column using acetonitrile-methanol-water as a mobile phase.
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