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Updated: Jul 28, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using
F A Drobniewski1, P G More, G S Harris
1PHLS Mycobacterium Reference Unit, Department of Microbiology, Dulwich Public Health Laboratory, Guy's King's and St Thomas' Medical School, Dulwich Hospital, London SE22 8QF, United Kingdom. francis.drobniewski@kcl.ac.uk
Abstract:
A blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81. 5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with either probe and were identified as Mycobacterium xenopi, Mycobacterium fortuitum, or Mycobacterium flavescens.
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