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Ca2+-activated non-selective cation current in rabbit ventricular myocytes.

Y Wu1, M E Anderson

  • 1The Cardiac Arrhythmia Section, Division of Cardiovascular Medicine, Department of Internal Medicine, Vanderbilt University Medical School, Nashville, TN 37232-6300, USA.

The Journal of Physiology
|January 5, 2000
PubMed
Summary

Oscillatory currents in rabbit ventricular myocytes depend on calcium release from the sarcoplasmic reticulum. These currents involve both calcium-activated chloride and non-selective cation channels, contributing to cardiac electrical activity.

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Area of Science:

  • Cardiology
  • Electrophysiology
  • Molecular Biology

Background:

  • Oscillatory currents (OCs) play a role in cardiac electrophysiology.
  • Understanding the ionic basis of OCs is crucial for comprehending cardiac function.

Purpose of the Study:

  • To investigate the ionic mechanisms underlying oscillatory currents in isolated rabbit ventricular myocytes.
  • To determine the contribution of different ion channels to OCs under specific experimental conditions.

Main Methods:

  • Whole-cell voltage clamp technique in isolated rabbit ventricular myocytes.
  • Utilized Na+-free solutions to isolate specific ionic currents.
  • Employed pharmacological agents like ryanodine, thapsigargin, and niflumic acid to block specific channels and calcium release.

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Main Results:

  • OCs were dependent on intracellular calcium release from the sarcoplasmic reticulum.
  • OCs involved both calcium-activated chloride current (ICl(Ca)) and calcium-activated non-selective cation current (ICAN).
  • Niflumic acid (ICl(Ca) antagonist) and N-methyl-D-glucamine (monovalent cation substitution) helped elucidate the contributions of ICAN and ICl(Ca).

Conclusions:

  • Oscillatory currents in rabbit ventricular myocytes are mediated by a combination of ICl(Ca) and ICAN.
  • Calcium release from the sarcoplasmic reticulum is essential for generating these currents.
  • ICAN contributes partially to the macroscopic oscillatory currents observed in these cells.