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Two murine monoclonal antibodies against serogroup E Salmonellae
1Departments of Microbiology, University of Hong Kong, Queen Mary Hospital Compound, Hong Kong, People's Republic of China. spng@hkusub.hku.hk
Abstract:
A monoclonal antibody (MAb), MO15, was raised against the lipopolysaccharide antigen of an epsilon15-lysogenized serogroup E(1) Salmonella strain. The O factor 15-specific MAb MO15, together with another serogroup E-specific MAb, can differentiate among phage lysogenization variants in serogroup E salmonellae. Their epitope specificities in relation to conventional O-antigenic structures are discussed.
Insights
A new monoclonal antibody, MO15, specifically targets the O factor 15 antigen in Salmonella. This antibody aids in distinguishing between different phage lysogenization variants within serogroup E Salmonella strains.
Area of Science:
- Immunology
- Microbiology
- Bacteriology
Background:
- Salmonella serogroup E strains exhibit variations due to phage lysogenization.
- Lipopolysaccharide (LPS) antigens are key surface structures on Salmonella.
- Differentiating Salmonella variants is crucial for epidemiological and diagnostic purposes.
Purpose of the Study:
- To develop a specific monoclonal antibody (MAb) for identifying Salmonella O factor 15.
- To investigate the utility of MAb MO15 in distinguishing phage lysogenization variants of serogroup E Salmonella.
- To analyze the epitope specificities of MAbs in relation to Salmonella O-antigenic structures.
Main Methods:
- Generation of a monoclonal antibody (MAb) designated MO15 against the LPS of a specific Salmonella strain.
- Utilizing MAb MO15 and another serogroup E-specific MAb for variant differentiation.
- Characterization of epitope specificities.
Main Results:
- MAb MO15 was successfully raised against the O factor 15 antigen of a lysogenized Salmonella strain.
- MAb MO15, in conjunction with a serogroup E-specific MAb, demonstrated the ability to differentiate phage lysogenization variants.
- The study discusses the epitope specificities of these MAbs concerning O-antigenic structures.
Conclusions:
- MAb MO15 is a valuable tool for the specific identification of the O factor 15 antigen in Salmonella.
- The combined use of serogroup E-specific MAbs provides a method for differentiating Salmonella lysogenization variants.
- Understanding MAb epitope specificities enhances the characterization of Salmonella O-antigens.