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Molecular diversity of Renibacterium salmoninarum isolates determined by randomly amplified polymorphic DNA analysis
T H Grayson1, F A Atienzar, S M Alexander
1Department of Biological Sciences, University of Plymouth, Plymouth PL4 8AA, Devon, United Kingdom. tgrayson@plymouth.ac.uk
Abstract:
The molecular diversity among 60 isolates of Renibacterium salmoninarum which differ in place and date of isolation was investigated by using randomly amplified polymorphic DNA (RAPD) analysis. Isolates were grouped into 21 banding patterns which did not reflect the biological source. Four 16S-23S rRNA intergenic spacer (ITS1) sequence variations and two alleles of an exact tandem repeat locus, ETR-A, were the bases for formation of distinct groups within the RAPD clusters. This study provides evidence that the most common ITS1 sequence variant, SV1, possesses two copies of a 51-bp repeat unit at ETR-A and has been widely dispersed among countries which are associated with mainstream intensive salmonid culture.