Function of the cytoskeleton in human neutrophils and methods for evaluation

The cytoskeleton plays a critical role in the determination of cell shape and serves as a scaffold for critical cellular enzymes and adhesion molecules. It provides structural integrity for the cell and regulates the function of many biochemical events that are critical to cellular function. The microfilamentous cytoskeleton participates in force generation necessary for shape change and motion. In neutrophils and other motile cells, polymerization of actin likely drives extension of the lamellae and participates in force generation through interaction with myosin, by polymerization alone and by osmotic mechanisms. Here, we will focus on the microfilamentous cytoskeleton in the neutrophil and briefly review its function as well as some direct and indirect methods that have been used to asses its role in neutrophil function. The discussion will address general approaches and leaves the details of the methods to the references.