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Association between plasma CC16 levels, the A38G polymorphism, and asthma
I A Laing1, C Hermans, A Bernard
1Department of Paediatrics, University of Western Australia, Children's Hospital Medical Centre, Perth, Australia. ingrid@ichr.uwa.edu.au
American Journal of Respiratory and Critical Care Medicine
|January 5, 2000
Summary
The A38G polymorphism in the Clara cell secretory protein (CC16) gene is linked to lower CC16 levels. Lower CC16 levels increase asthma risk in children, suggesting CC16
Area of Science:
- Genetics and Respiratory Medicine
- Molecular Biology and Immunology
Background:
- Asthma is a complex respiratory disease with genetic and environmental factors.
- Clara cell secretory protein (CC16) is a protein produced by lung cells, and its levels may be associated with asthma.
- The A38G polymorphism in the CC16 gene has been proposed as a potential risk factor for asthma.
Purpose of the Study:
- To investigate the association between the A38G polymorphism of the CC16 gene and asthma in children.
- To determine if plasma CC16 levels are affected by the A38G polymorphism and if they correlate with asthma status.
Main Methods:
- A study involving 100 asthmatic and non-asthmatic children.
- Genotyping for the A38G polymorphism using restriction digestion.
- Quantification of plasma CC16 levels via latex immunoassay.
Main Results:
- Asthmatic children exhibited significantly lower mean plasma CC16 levels compared to non-asthmatic children.
- Individuals with the 38A/38A genotype had lower plasma CC16 levels than those with the 38G/38G genotype.
- The 38A/38A genotype was associated with an increased odds ratio for asthma, an effect largely mediated by reduced plasma CC16 levels.
Conclusions:
- The 38A allele of the CC16 gene is associated with reduced plasma CC16 levels.
- Lower plasma CC16 levels are a significant risk factor for asthma development in children.
- These findings highlight the role of the CC16 gene and its polymorphisms in asthma pathogenesis.