Wide-field optically sectioning fluorescence microscopy with laser illumination
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Updated: Aug 14, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
M A Neil1, A Squire, R Juskaitis
1Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, U.K.
Abstract:
We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.
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