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Related Experiment Videos

Cell volume measurement using scanning ion conductance microscopy.

Y E Korchev1, J Gorelik, M J Lab

  • 1Division of Medicine, Imperial College School of Medicine, Medical Research Council Clinical Sciences Centre, Hammersmith Campus, Du Cane Road, London W12 0NN, United Kingdom. y.korchev@ic.ac.uk

Biophysical Journal
|January 5, 2000
PubMed
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We developed a new scanning ion conductance microscopy (SICM) method to measure living cell volumes and dynamic changes with high resolution. This technique requires no sample preparation and can assess cellular structures and responses to stimuli.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Accurate measurement of living cell volume is crucial for understanding cellular processes.
  • Existing techniques may require sample preparation or lack the resolution for dynamic changes.
  • High-resolution, non-invasive methods are needed to study cell volume dynamics.

Purpose of the Study:

  • To introduce a novel scanning ion conductance microscopy (SICM) technique for quantitative cell volume assessment.
  • To demonstrate the capability of SICM for high-resolution, dynamic cell volume measurements.
  • To validate SICM for assessing cellular structures and responses to physiological stimuli.

Main Methods:

  • Utilized scanning ion conductance microscopy (SICM) for non-invasive cell volume measurement.

Related Experiment Videos

  • Developed a method for quantitative assessment of cell and subcellular structure volumes.
  • Performed continuous, long-term monitoring of cell volume and surface characteristics.

    • Main Results:

    • Achieved quantitative, high-resolution measurement of living cell volumes (10^-19 to 10^-9 L).
    • Demonstrated resolution of 2.5 x 10^-20 L for cell volume and subcellular structures.
    • Showcased simultaneous assessment of cell volume and surface characteristics without sample preparation.
    • Validated rapid estimation of cell volume changes in response to stimuli.

    Conclusions:

    • The novel SICM technique provides a powerful tool for quantitative, high-resolution analysis of living cell volumes.
    • This method allows for continuous monitoring of dynamic cellular changes and responses.
    • SICM offers a versatile, non-invasive approach for cell biology research and physiological studies.