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Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of
Q X Zhang1, C S Pilquil, J Dewald
1Department of Biochemistry, Lipid and Lipoprotein Research Group, University of Alberta, 357 Heritage Medical Research Centre, Edmonton, Alberta, Canada T6G 2S2.
The Biochemical Journal
|January 6, 2000
Abstract:
Lipid phosphate phosphatase-1 (LPP-1) dephosphorylates exogenous lysophosphatidate and thereby regulates the activation of lysophosphatidate receptors and cell division. Mutation of seven amino acids in three conserved domains of mouse LPP-1 abolished its activity. A glycosylation site was demonstrated between conserved Domains 1 and 2. LPP-1 is expressed in the plasma membrane, and the present results demonstrate the active site to be located on the outer surface.