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Peripheral blood stem cell collection and transplantation using the Haemonetics Multi Component System.
Transfusion Science
|January 6, 2000
Summary
This study shows an intermittent-flow blood cell separator effectively collects peripheral blood stem cells (PBSC). High CD34+ cell percentages predict successful PBSC collection for transplantation.
Area of Science:
- Hematology
- Transplantation Medicine
- Biomedical Engineering
Background:
- Peripheral Blood Stem Cell (PBSC) collection is crucial for transplantation.
- Assessing the efficacy of new apheresis technologies is vital for improving patient outcomes.
Purpose of the Study:
- To evaluate the clinical utility of an intermittent-flow blood cell separator for PBSC collection.
- To determine the predictive value of CD34+ cell counts for collection efficiency.
Main Methods:
- Utilized the Haemonetics Multi Component System (Multi) for 52 apheresis procedures in 17 patients.
- Monitored processing blood volume and PBSC yield (CD34+ cells/kg).
Main Results:
- Mean processing volume was 7407 ml, with a mean PBSC yield of 2.16 x 10^6 CD34+ cells/kg.
- Collections exceeding 2.0 x 10^6 CD34+ cells/kg were achievable in a single apheresis when CD34+ cells were >0.3% of peripheral white blood cells (WBC).
- Eight patients received PBSC transplantation, achieving hematopoietic recovery in a median of 10 days.
Conclusions:
- A single-arm, lightweight blood cell separator demonstrates adequate capability for PBSC collection.
- Peripheral blood CD34+ cell percentage serves as a reliable predictor for successful PBSC yield during apheresis.