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Mixed phenotype lymphomas in thymectomized (SL/KhxAKR/Ms)F1 mice
1Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University.
Japanese Journal of Cancer Research : Gann
|January 6, 2000
Summary
Thymectomy in mice altered lymphoma development, shifting from T-cell to unusual mixed-phenotype lymphomas. These lymphomas, resembling large granular lymphocytes, grew similarly in thymectomized and intact mice.
Area of Science:
- Immunology
- Oncology
- Genetics
Background:
- Lymphomagenesis in mice involves genetic and epigenetic factors.
- Specific mouse strains (SL/Kh and AKR/Ms) develop distinct lymphoma types (pre-B and T-lymphomas, respectively).
- A dominant gene, Tlsm1, from AKR mice dictates T-lymphoma development in hybrid crosses.
Purpose of the Study:
- To investigate the role of the Tlsm1 gene in determining lymphoma type.
- To examine the effect of early-life thymectomy on lymphoma development in (SL/KhxAKR/Ms)F1 hybrid mice.
Main Methods:
- Thymectomy was performed on 1-week-old (SL/KhxAKR/Ms)F1 hybrid mice.
- Lymphoma phenotype analysis included cell surface markers (CD5, NK1, Mac1), gene rearrangement, and morphology.
- Histochemistry, electron microscopy, and cytokine expression (interleukin-4, perforin, interferon-gamma) were utilized.
- Transplantation studies assessed lymphoma growth in recipient mice.
Main Results:
- Intact F1 mice predominantly developed T-lineage lymphomas.
- Thymectomized F1 mice developed unusual mixed-phenotype lymphomas.
- These mixed-phenotype lymphomas exhibited characteristics of B-cell lymphomas with features of large granular lymphocytes, expressing NK1, Mac1, acid phosphatase, and lysozyme.
- Transplanted lymphoma cells showed similar growth rates in both intact and thymectomized recipients.
Conclusions:
- Early thymectomy significantly alters lymphoma development in the presence of the Tlsm1 gene.
- The study identifies a novel mixed-phenotype lymphoma with characteristics of large granular lymphocytes.
- The thymus environment influences lymphoma cell lineage determination, but the Tlsm1 gene's effects on lymphoma growth post-thymectomy require further investigation.