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A role for ADP ribosylation factor in the control of cargo uptake during COPI-coated vesicle biogenesis

J Malsam1, D Gommel, F T Wieland

  • 1Biochemie Zentrum Heidelberg, Ruprecht Karls-Universität Heidelberg, Germany.

FEBS Letters
|January 6, 2000
PubMed

Insights

ADP-ribosylation factor (ARF) GTPase regulates both coat disassembly and cargo uptake in COPI vesicle transport. This study reveals ARF

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • ADP-ribosylation factor (ARF) GTPase is known to regulate COPI vesicle coat disassembly.
  • GTP hydrolysis is crucial for COPI vesicle biogenesis, as indicated by impaired cargo uptake with GTPgammaS.

Purpose of the Study:

  • To identify the specific GTPase regulating cargo uptake in COPI vesicle biogenesis.
  • To elucidate the multi-functional role of ARF in COPI-mediated vesicular transport.

Main Methods:

  • Investigated the role of GTPase in COPI vesicle cargo uptake.
  • Utilized GTPgammaS, a non-hydrolyzable GTP analogue, to assess cargo uptake impairment.

Main Results:

  • Demonstrated that ARF is the GTPase responsible for regulating cargo uptake.
  • Established a multi-functional role for ARF in COPI vesicle transport, impacting both cargo uptake and coat disassembly.

Conclusions:

  • ARF plays a critical role in multiple stages of COPI vesicle biogenesis.
  • Understanding ARF's function provides insights into the molecular mechanisms of vesicular transport.

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