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[Preparation of 32P-labelled 2-methylthioadenosine di- and triphosphates]
M Iu Skoblov1, M V Ias'ko, Iu S Skoblov
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Bioorganicheskaia Khimiia
|January 7, 2000
Abstract:
2-Methylthioadenosine 5'-mono- and diphosphates were prepared by chemical synthesis, and enzymatically phosphorylated to 5'-[beta-32P]-diphosphate and 5'-[gamma-32P]-triphosphate, respectively. They were isolated by HPLC and had activity exceeding 1000 Ci/mmol and radiochemical purity higher than 95%.