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[The transfer of foreign DNA by rabbit spermatozoa into hamster eggs]
A V Kuznetsov1, I Iu Shchit, I V Kuznetsova
1State Scientific Center of Applied Microbiology, Obolensk, Moscow Oblast, Russia.
Ontogenez
|January 7, 2000
Abstract:
Capacitated rabbit sperm was incubated with pCMVlacZ plasmid and then used to fertilize hamster oocytes liberated from zona pellucida. After treatment with DNase I, these oocytes were examined for the presence of exogenous DNA by PCR. DNA of pCMVlacZ was not found in oocytes after simple incubation with male gametes. The presence of DNA was recorded in the experiments where the sperms were treated with DMSO and subjected to heat shock prior to fertilization.