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Chemokine involvement in tetracycline-induced pleuritis
E J Miller1, O Kajikawa, S Pueblitz
1Dept of Biochemistry, The University of Texas Health Center at Tyler, 75708-3154, USA.
The European Respiratory Journal
|January 7, 2000
Summary
Tetracycline (TCN) instillation in malignant pleural effusions triggers an inflammatory response. Interleukin-8 (IL-8) and growth-related protein (Gro) from mesothelial cells may drive neutrophil influx during TCN-induced pleuritis.
Area of Science:
- Pulmonary Medicine
- Cellular Biology
- Immunology
Background:
- Tetracycline (TCN) is used to control malignant pleural effusions.
- The cellular signals driving inflammation after TCN instillation are not fully understood.
Purpose of the Study:
- To investigate the role of chemokines, specifically interleukin-8 (IL-8), growth-related protein (Gro), and monocyte chemotactic protein-1 (MCP-1), in the pleural space following intrapleural TCN administration within the first 72 hours.
Main Methods:
- Intrapleural TCN administration in a model of malignant pleural effusion.
- Analysis of pleural fluid for leukocyte content, including neutrophils and macrophages.
- Measurement of chemokine concentrations (IL-8, Gro, MCP-1) over time.
- Immunocytochemical analysis of IL-8 expression in pleural mesothelial cells.
Main Results:
- TCN induced an exudative effusion with high lactate dehydrogenase.
- Polymorphonuclear neutrophil concentration decreased, while macrophage concentration increased between 24 and 72 hours post-TCN.
- Concentrations of IL-8, Gro, and MCP-1 decreased between 24 and 72 hours.
- IL-8 expression was observed in pleural mesothelial cells at 24 hours but not at 72 hours.
Conclusions:
- Local production of IL-8 and Gro, partly from mesothelial cells, may contribute to neutrophil recruitment in tetracycline-induced pleuritis.
- These findings shed light on the inflammatory mechanisms involved in TCN sclerotherapy for malignant pleural effusions.