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Related Experiment Videos

Current-source-density profiles associated with sharp waves in human epileptic neocortical tissue.

R Köhling1, M Qü, K Zilles

  • 1Institut für Physiologie, Westfälische Wilhelms-Universität, Münster, Germany. kohling@uni-muenster.de

Neuroscience
|January 7, 2000
PubMed
Summary

This study reveals that spontaneous epileptic discharges in human neocortical slices originate in superficial layers (II and III). Bicuculline application alters this origin, highlighting functional changes in these layers during epilepsy.

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Area of Science:

  • Neuroscience
  • Epileptology
  • Human tissue research

Background:

  • Spontaneous sharp waves, characteristic of epileptiform activity, are observed in human neocortical slices from epilepsy surgery.
  • Understanding the underlying current sources and sinks of these potentials is crucial for epilepsy research.

Purpose of the Study:

  • To analyze the current sinks and sources responsible for spontaneous epileptiform field potentials in human neocortical slices.
  • To investigate the impact of altered ionic concentrations (zero Mg2+) and pharmacological agents (bicuculline) on epileptiform activity.

Main Methods:

  • Utilized human neocortical tissue from temporal, frontal, and parietal lobes obtained during epilepsy surgery.
  • Recorded field potentials using a linear array of electrodes in 500-micrometer thick slices.

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  • Classified slices as spontaneous or non-spontaneous based on observed epileptiform discharges.

    • Main Results:

    • In spontaneous slices, current sinks for interictal discharges were primarily in layers II and III.
    • Ictal activity in spontaneous slices initiated in layers II and IIIa, shifting deeper with bicuculline.
    • Non-spontaneous slices required bicuculline to induce ictal activity, with sinks also in layers II and IIIa.

    Conclusions:

    • Supragranular layers, particularly layer II, exhibit altered functional organization in slices with spontaneous discharges.
    • These findings suggest a critical role for superficial cortical layers in the initiation and propagation of epileptiform activity.
    • Bicuculline's effect indicates its ability to modulate the functional organization and discharge initiation zones within the neocortex.