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Related Experiment Videos

Development and application of cell-based biosensors.

J J Pancrazio1, J P Whelan, D A Borkholder

  • 1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA. jxp@cbmse.nrl.navy.mil

Annals of Biomedical Engineering
|January 7, 2000
PubMed
Summary

Cell-based biosensors offer unique physiological insights for analyte detection. Networks of excitable cells show promise, but challenges in methods, reproducibility, and cell sources need addressing for wider application.

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Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Cellular Engineering

Background:

  • Biosensors utilize biological sensing elements for signal conversion.
  • Applications span environmental monitoring to defense.
  • Key characteristics include analyte selectivity and physiological responsiveness.

Purpose of the Study:

  • To review molecular and cell-based biosensors.
  • To discuss signal transduction approaches in cell-based biosensors.
  • To identify challenges and future solutions for cell-based biosensor applications.

Main Methods:

  • Review of molecular biosensors (antibodies, enzymes, ion channels, nucleic acids).
  • Discussion of cell-based biosensors using microorganisms and mammalian cells.

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  • Exploration of signal transduction methods: cell metabolism, impedance, potentials.

    • Main Results:

    • Cell-based biosensors, particularly using mammalian cells, provide physiological effect insights.
    • Networks of excitable cells on microelectrode arrays show potential for rapid analyte classification.
    • Identified key challenges: analytical methods, reproducibility, and cell sources.

    Conclusions:

    • Cell-based biosensors, especially using excitable cell networks, represent a promising technology.
    • Overcoming challenges in analytical methods, reproducibility, and cell sourcing is crucial for advancement.
    • Future research should focus on addressing these barriers to enhance cell-based biosensor utility.