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Related Experiment Videos

Delayed-type hypersensitivity reaction induced in broilers by killed Staphylococcus aureus.

X Y Zhu1, R E Porter, P Y Hester

  • 1Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA.

Poultry Science
|January 8, 2000
PubMed
Summary
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The delayed footpad reaction (DFR) in chickens challenged with Staphylococcus aureus is a reliable indicator of cell-mediated delayed-type hypersensitivity (DTH). This study confirms DFR as a valuable in vivo test for assessing DTH responses in poultry.

Area of Science:

  • Immunology
  • Veterinary Medicine
  • Cellular Biology

Background:

  • Delayed-type hypersensitivity (DTH) is a critical immune response.
  • Assessing DTH in chickens is important for understanding immune function.
  • The delayed footpad reaction (DFR) is a potential indicator of DTH.

Purpose of the Study:

  • To determine if the delayed footpad reaction (DFR) induced by killed Staphylococcus aureus in chickens represents a delayed-type hypersensitivity (DTH) reaction.
  • To validate the DFR as a standard in vivo test for cell-mediated immunity in poultry.

Main Methods:

  • Chickens were sensitized with Staphylococcus aureus antigen and challenged intradermally in the footpad.
  • Delayed footpad reaction (DFR) was measured by footpad thickness.

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  • Lymphocyte blastogenesis, monocyte migration inhibition, histology, and passive transfer of DTH were assessed.

    • Main Results:

    • Sensitized chickens exhibited a significantly increased DFR and lymphocyte blastogenic response.
    • In vitro monocyte/macrophage migration was inhibited in sensitized birds.
    • Histology revealed mononuclear cell infiltration in challenged footpads.
    • Passive transfer of splenic lymphocytes from sensitized birds induced a pronounced DFR in recipients.

    Conclusions:

    • The delayed footpad reaction (DFR) in chickens meets the criteria for a delayed-type hypersensitivity (DTH) response.
    • DFR is a validated in vivo test for assessing cell-mediated immunity induced by Staphylococcus aureus antigen in chickens.
    • This finding supports the use of DFR in poultry immunology research and diagnostics.