Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel method for assigning TAP1 genotype using restriction enzyme plus PASA methodology.

H Shitaye1, M S Hayney, D W Love

  • 1Mayo Vaccine Research Group, Clinical Pharmacology Unit, Mayo Clinic and Foundation, Rochester, MN 55905, USA.

Human Immunology
|January 8, 2000
PubMed
Summary

We developed a new method for definitive TAP1 genotyping, especially for complex TAP1U cases. This improves accuracy and efficiency in large-scale human disease-gene association studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to: Use of statins in patients with COVID-19.

QJM : monthly journal of the Association of Physicians·2020
Same author

Does apolipoprotein E genotype predict COVID-19 severity?

QJM : monthly journal of the Association of Physicians·2020
Same author

Response to: Cardiac drugs and outcome in COVID-19.

QJM : monthly journal of the Association of Physicians·2020
Same author

Are certain drugs associated with enhanced mortality in COVID-19?

QJM : monthly journal of the Association of Physicians·2020
Same author

Usability of daily SF36 questionnaires to capture the QALD variation experienced after vaccination with AS03<sub>A</sub>-adjuvanted monovalent influenza A (H5N1) vaccine in a safety and tolerability study.

Health and quality of life outcomes·2019
Same author

Knowledge gaps persist and hinder progress in eliminating mumps.

Vaccine·2018

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Human Genetics

Background:

  • The transporter associated with antigen processing (TAP) gene products are crucial for antigen presentation via HLA molecules.
  • TAP gene polymorphisms are studied for their potential association with various diseases.
  • Existing TAP1 genotyping methods struggle with definitive assignment in individuals with multiple polymorphic positions, particularly the TAP1U combination.

Purpose of the Study:

  • To develop a reliable method for definitive TAP1 genotyping, especially for individuals with the TAP1U combination.
  • To optimize existing genotyping techniques for improved efficiency and cost-effectiveness.
  • To facilitate large-scale human disease-gene association studies by enabling rapid and accurate TAP genotyping.

Main Methods:

Related Experiment Videos

  • Development of a novel restriction enzyme-based method for definitive TAP1 genotype assignment.
  • Optimization and modification of PCR amplification of specific alleles (PASA) for TAP genotyping.
  • Application of these methods to accurately genotype individuals, including those with the TAP1U combination.

Main Results:

  • The novel restriction enzyme method achieves 100% definitive TAP1 genotype assignment for all subjects, including TAP1U cases.
  • Modified PASA reactions offer significant time and cost savings for TAP genotyping.
  • The developed methods provide rapid and efficient assignment of TAP genotypes.

Conclusions:

  • A new restriction enzyme-based method and optimized PASA reactions enable definitive TAP1 genotyping, particularly for TAP1U subjects.
  • These advancements are crucial for accurate and efficient genetic analysis in large human populations.
  • The improved genotyping techniques will significantly aid in large-scale human disease-gene association studies.