Cell cycle components and their potential impact on the development of continuous in vitro penaeid cell replication

In vitro prawn cell culture has yet to produce an established cell line. In an effort to establish some understanding of the cellular blockage that prohibits their division in vitro we conducted several studies to characterize the cytoskeletal components of hemocytes and found no cells undergoing mitosis. Following this discovery, a molecular analysis of cell division regulatory proteins was performed. Cell cycle regulatory proteins (cyclins) have been identified as essential components in the progression of all eukaryotic cells through the cell cycle. We report here the identification of cyclin A and cyclin B proteins and their cofactor (p34(cdc2)) in making up the mitosis promoting factor (MPF) in protein extracts from egg and muscle tissues of Penaeus vannamei. Molecular weight analysis confirmed the size of the target proteins to be similar to the same proteins identified in the Atlantic surf clam (Spisula solidissima).