Related Experiment Video
Updated: Aug 27, 2026

An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation
Published on: June 30, 2015
Sporostatin, a novel and specific inhibitor of EGF receptor kinase
Y Murakami1, A Ishii, S Mizuno
1Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan.
Abstract:
Sporostatin isolated from a fungus of Sporormiella sp.M5032 as an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase, was found to be a specific inhibitor of epidermal growth factor (EGF) receptor tyrosine kinase in vitro. Its IC50 values were 0.1 microgram/ml (0.38 microM) for EGF receptor kinase, 3 micrograms/ml (11 microM) for ErbB-2, and 100 micrograms/ml (380 microM) or more than that for other kinases including PDGF receptor, v-src and protein kinase C. Kinetic analyses revealed that inhibition of EGF receptor kinase by sporostatin was noncompetitive either with substrate or with ATP. Autophosphorylation of EGF receptor in A431 cells was also inhibited. These results show that sporostatin is a potent and specific inhibitor of EGF receptor kinase.
More Related Videos
Related Concept Videos
Enzyme-linked Receptors
Mitogens and the Cell Cycle
Amplifying Signals via Enzymatic Cascade
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:

