Improved metastatic animal model of human prostate carcinoma using surgical orthotopic implantation (SOI)

An animal model demonstrating high metastasis of human prostate carcinoma is of importance in studying the biology and therapy of human prostate carcinoma Dr. Hoffman's group recently used surgical orthotopic implantation (SOI) of human prostate cells to nude mice to establish an animal model with high metastatic activity. To confirm the animal model by SOI reproducible in other laboratories and shorten the period requiring for the metastasis, we adopted SOI technique with a modification using PC-3M human prostate cell line which showed a higher metastatic activity than PC-3.

Intact tissue of the human prostate carcinoma cell line PC-3M was prepared by growth of this cell subcutaneous in a nude mouse. One piece of 1.5 mm3 intact tumor tissue was implanted by orthotopical surgery to the ventral lateral lobes of the prostate gland of 10 nude mice. Mice were sacrificed when they were found to be moribund. Metastasis in other tissues was evaluated by gross and microscopic morphology.

All 10 mice showed the tumorigenesis in the prostate gland and metastasis of human prostate tumor cells into periaortic lymph nodes without other organ's metastasis. The time when mice with PC-3M SOI start moribund is 28-32 days after SOI.

SOI is good technique to establish the efficiently metastatic animal mode. SOI using PC-3M human prostate cell line will leads to 100% metastasis of prostate tumor cells. So far, this model is much quicker and more efficient than those reported in literatures.