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GPI-anchored molecule-like protein (GML) expression in non-small cell lung cancer (NSCLC)
T Komiya1, T Hirashima, M Kikui
1Department of Internal Medicine, Osaka Prefectural Habikino Hospital, Japan.
Background:
GPI-anchored molecule-like protein (GML) has been identified as a new target molecule of p53. In esophageal cancer cell lines, expression of GML mRNA, which was found to be significantly correlated with in vitro anti-cancer drug sensitivity, was observed only in the presence of wild-type p53.
Materials And Methods:
The relationship between expression of GML mRNA and p53 gene status in 24 non-small cell lung cancer (NSCLC) cell lines was examined using RT-PCR and PCR-SSCP followed by direct DNA sequencing.
Results:
Expression of GML mRNA was observed in only 3 of 24 cell lines. In contrast to previous studies showing p53-dependent GML expression, of the 3 cell lines expressing GML mRNA, one had a p53 gene mutation (codon 245: Gly to Cys).
Conclusion:
These findings suggest that the expression of GML mRNA is infrequent and regulated in a p53-independent manner in NSCLC.
Insights
GPI-anchored molecule-like protein (GML) expression is infrequent in non-small cell lung cancer (NSCLC) and appears to be regulated independently of the p53 gene, contrary to previous findings in other cancers.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- GPI-anchored molecule-like protein (GML) is a novel p53 target.
- GML mRNA expression correlates with drug sensitivity in esophageal cancer.
- GML expression was previously thought to be p53-dependent.
Purpose of the Study:
- To investigate the relationship between GML mRNA expression and p53 gene status in non-small cell lung cancer (NSCLC).
Main Methods:
- Analysis of 24 NSCLC cell lines.
- Reverse transcription-polymerase chain reaction (RT-PCR) for GML mRNA detection.
- Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing for p53 gene status.
Main Results:
- GML mRNA expression was detected in only 3 out of 24 NSCLC cell lines.
- One of the GML-expressing cell lines harbored a p53 gene mutation (codon 245).
- This contrasts with previous studies suggesting p53-dependent GML expression.
Conclusions:
- GML mRNA expression is infrequent in NSCLC.
- GML mRNA expression in NSCLC is regulated in a p53-independent manner.