Related Experiment Videos
Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on
G Künzel1, L Korzun, A Meister
1Institut f]ur Pflanzengenetik und Kulturpflanzenforschung (IPK), 06466 Gatersleben, Germany. kuenzel@ipk-gatersleben.de
Abstract:
We have developed a new technique for the physical mapping of barley chromosomes using microdissected translocation chromosomes for PCR with sequence-tagged site primers derived from >300 genetically mapped RFLP probes. The positions of 240 translocation breakpoints were integrated as physical landmarks into linkage maps of the seven barley chromosomes. This strategy proved to be highly efficient in relating physical to genetic distances. A very heterogeneous distribution of recombination rates was found along individual chromosomes. Recombination is mainly confined to a few relatively small areas spaced by large segments in which recombination is severely suppressed. The regions of highest recombination frequency (