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Macrolide resistance in Belgian Streptococcus pneumoniae.

K Lagrou1, W E Peetermans, J Verhaegen

  • 1Departments of Microbiology and Immunology, Rega Institute for Medical Research, K.U. Leuven, Belgium. katrien.lagrou@rega.kuleuven.ac.be

The Journal of Antimicrobial Chemotherapy
|January 11, 2000
PubMed
Summary

Macrolide resistance in Streptococcus pneumoniae was studied. The ermAM gene caused most resistance, but mefE-mediated resistance, common in North America, was rare in Belgium.

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Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Streptococcus pneumoniae is a leading cause of invasive bacterial infections.
  • Macrolide resistance in S. pneumoniae is a growing public health concern.
  • Understanding the genetic basis of resistance is crucial for effective treatment.

Purpose of the Study:

  • To characterize the genetic basis of macrolide resistance in Streptococcus pneumoniae isolates from Belgium.
  • To determine the prevalence of specific resistance genes (ermAM and mefE) in these isolates.
  • To compare macrolide resistance patterns in Belgium with those in Canada and the USA.

Main Methods:

  • Collection of 59 S. pneumoniae isolates from invasive infections between 1995 and 1997.
  • Phenotypic characterization of macrolide resistance (erythromycin and clindamycin).

Related Experiment Videos

  • Molecular detection of ermAM and mefE genes using Polymerase Chain Reaction (PCR).

    • Main Results:

    • The majority of isolates (54/59) exhibited the MLS(B)-phenotype and carried the ermAM gene.
    • Five isolates displayed the M-phenotype (erythromycin resistant, clindamycin susceptible) and harbored the mefE gene.
    • mefE-mediated erythromycin resistance in S. pneumoniae was found to be uncommon in Belgium.

    Conclusions:

    • The ermAM gene is the predominant mechanism of macrolide resistance in S. pneumoniae from invasive infections in Belgium during 1995-1997.
    • The mefE gene, a common cause of erythromycin resistance in North America, is infrequent in Belgian S. pneumoniae isolates.
    • These findings highlight regional variations in macrolide resistance determinants in S. pneumoniae.