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The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices,
1Department of Microbiology, University of Western Ontario, London, Ontario N6A 5C1, Canada.
Journal of Bacteriology
|January 12, 2000
Abstract:
The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.