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High-performance liquid chromatography separation methods for the analysis of peptide nucleic acids
1Department of Chemistry, American University, Washington, DC 20016, USA.
Journal of Chromatography. A
|January 12, 2000
Abstract:
An analytical analysis of peptide nucleic acids (PNAs) was carried out by reversed-phase HPLC using a solvent system comprised of aqueous trifluoroacetic acid and acetonitrile. A regression equation was obtained which represents the relationship of the molecular mass, sequence composition and retention time. This equation can be used to estimate the retention time of a known PNA under certain HPLC conditions. In addition to this equation, new HPLC conditions were also optimized which can be used for separation of pure PNAs.