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Related Experiment Videos

Human vitamin C (L-ascorbic acid) transporter SVCT1.

Y Wang1, B Mackenzie, H Tsukaguchi

  • 1Membrane Biology Program and Renal Division, Department of Pathology, Department of Obstetrics, Gynecology & Reproductive Biology, Brigham & Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.

Biochemical and Biophysical Research Communications
|January 13, 2000
PubMed
Summary

Scientists identified two human vitamin C transporters, SVCT1 and SVCT2. These transporters are crucial for regulating vitamin C levels and understanding its role in health and disease.

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Area of Science:

  • Molecular Biology
  • Human Physiology
  • Nutritional Science

Background:

  • Vitamin C (l-ascorbic acid) is an essential micronutrient vital for numerous biological functions in humans, including enzymatic reactions and antioxidant defense.
  • Understanding the molecular mechanisms of vitamin C transport is crucial for comprehending its metabolism and role in health and disease.

Purpose of the Study:

  • To identify and characterize novel human cDNA encoding vitamin C transporters.
  • To investigate the function, substrate specificity, and localization of these transporters.
  • To map the genes responsible for these transporters and explore their potential association with diseases.

Main Methods:

  • Molecular cloning of human cDNA.
  • Expression of transporters in Xenopus oocytes.

Related Experiment Videos

  • Radiotracer uptake assays and voltage-clamp electrophysiology.
  • Gene mapping using human chromosome analysis.

    • Main Results:

    • Molecular cloning of SVCT1, a novel human vitamin C transporter, primarily found in kidney and small intestine epithelia.
    • SVCT1 mediates saturable, high-affinity, electrogenic l-ascorbic acid transport, inhibited by phloretin, with high substrate selectivity.
    • The SLC23A2 gene (coding SVCT1) mapped to chromosome 5q31.2-31.3; SLC23A1 encodes SVCT2, a related transporter found in brain, retina, and endocrine tissues.

    Conclusions:

    • The molecular identification of human l-ascorbic acid transporters SVCT1 and SVCT2 provides essential tools for studying vitamin C metabolism.
    • These transporters play critical roles in maintaining vitamin C homeostasis in various tissues.
    • Further research can now focus on the implications of these transporters in human health and disease, including their potential link to leukemia.